Correction: Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations

This article has been published as a correction for an error in the manuscript of Pavlov et al BMC Biology 2004, 2:11. This article has been published as a correction for [1]. The legend for figure 2 in the original article states, "(C) Plot of time-course of DNA synthesis and dTMP turnover by wild-type Pol e. Open circles connected by solid line represent dTMP retained into DNA; open rectangles connected by a dashed line represent excised dTMP. (D) Plot of time-course of DNA synthesis and dTMP turnover by Y831A Pol e. Symbols are the same as in (C)." However, this is incorrect and should be revised to the following, "(C) Plot of time-course of DNA synthesis by wildtype Pol e (open circles connected by solid line) and Y831A Pol e (open rectangles connected by a dashed line). The data are derived from Fig 2A and are based on amount of dTMP incorporated into DNA. (D) Plot of time-course of dTMP generation by 3'->5' exonuclease activity by wild-type Pol e and Y831A Pol e (symbols are the same as in (C)). The data are derived from Fig 2B and based on amount of dTMP excised from DNA (turnover)." We apologise for any inconvenience or confusion that this may have caused. Here we have provided the original figure and the corrected legend, as figure 1. Published: 21 June 2007 BMC Biology 2007, 5:27 doi:10.1186/1741-7007-5-27 Received: 19 June 2007 Accepted: 21 June 2007 This article is available from: http://www.biomedcentral.com/1741-7007/5/27 © 2007 Pavlov et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Page 1 of 2 (page number not for citation purposes) BMC Biology 2007, 5:27http://www.biomedcentral.com/1741-7007/5/27 Publish with BioMed Central and everyscientist can read your work free of charge"BioMed Central will be the most significant development fordisseminating the results of biomedical research in our lifetime."Sir Paul Nurse, Cancer Research UKYour research papers will be:available free of charge to the entire biomedical communitypeer reviewed and published immediately upon acceptancecited in PubMed and archived on PubMed Centralyours — you keep the copyrightSubmit your manuscript here:http://www.biomedcentral.com/info/publishing_adv.aspBioMedcentralReferences1. YI Pavlov, S Maki, H Maki, TA Kunkel: Evidence for interplayamong yeast replicative DNA polymerases alpha, delta andepsilon from studies of exonuclease and polymerase activesite mutations. BMC Biology 2004, 2:11. This is the original image for figure 2, with the corrected ver-sion of the lege dFigure 1This is the original image for figure 2, with the cor-rected version of the legend. Elevated nucleotide turno-ver by Y831A Pol ε. DNA synthesis and dTTP turnover byPol ε was measured on poly(dA)/oligo(dT) substrate. Reac-tions were performed as described in Methods, using 0.13 Uof each enzyme for 20 μl reactions. (A) Analysis of polymer-ase reaction by TLC in 1 M LiCl running buffer. Lanes 1, 2, 3,4: wild-type Pol ε at 0, 3, 7 and 15 minutes of reaction,respectively. Lanes 5, 6, 7: reactions with Y831A Pol ε at 3, 7and 15 minutes, respectively. Positions of unincorporatedlabel, label in DNA and dTMP are shown by arrows. (B)Analysis of polymerase reaction by TLC in 0.4 M LiCl runningbuffer. Lane assignment is the same as in (A). (C) Plot oftime-course of DNA synthesis by wild-type Pol e (open cir-cles connected by solid line) and Y831A Pol e (open rectan-gles connected by a dashed line). The data are derived fromFig 2A and are based on amount of dTMP incorporated intoDNA. (D) Plot of time-course of dTMP generation by 3'->5'exonuclease activity by wild-type Pol e and Y831A Pol e(symbols are the same as in (C)). The data are derived fromFig 2B and based on amount of dTMP excised from DNA(turnover).A.B.